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TSCAN

TSCAN

TSCAN (Tools for Single Cell Analysis) is a tool for differential analysis of single-cell RNA-seq data. The TSCAN algorithm uses a cluster-based minimum spanning tree (MST) method for the pseudo-temporal ordering of cells. TSCAN package has a graphical user interface implemented in Shiny.

Topic

Transcriptomics; Gene expression; Bioinformatics; Data mining

Details

  • Operation: RNA-seq time series data analysis; Gene expression profiling; Gene expression comparison
  • Software interface: Command-line user interface; Graphical user interface; Library
  • Language: R;Shiny
  • Operating system: Linux; Mac OS X; Microsoft Windows
  • License: GNU General Public License v>=2
  • Cost: Free
  • Version name: 1.24.0
  • Maturity: Mature
  • Credit: National Institutes of Health (NIH).
  • Contact: Zhicheng Ji zji4 _at_ jhu.edu
  • Collection: BioConductor

Publications

Ji Z, Ji H "TSCAN: Pseudo-time reconstruction and evaluation in single-cell RNA-seq analysis." Nucleic Acids Res. 2016 Jul 27;44(13):e117. May 13. https://doi.org/10.1093/nar/gkw430
PMID: 27179027
PMCID: PMC4994863


Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J1, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M "Orchestrating high-throughput genomic analysis with Bioconductor." Nat Methods. 2015 Feb;12(2):115-21. https://doi.org/10.1038/nmeth.3252
PMID: 25633503
PMCID: PMC4509590


Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JY, Zhang J. "Bioconductor: open software development for computational biology and bioinformatics." Genome Biol. 2004;5(10):R80. Epub 2004 Sep 15. https://doi.org/10.1186/gb-2004-5-10-r80
PMID: 15461798
PMCID: PMC545600


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