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VDJPuzzle

VDJPuzzle

VDJPuzzle is a tool to reconstruct full-length B-cell receptors from single-cell RNA-seq data sets. The VDJPuzzle algorithm can reconstruct both heavy and light chains, and detect mutations in the V(D)J region.

Topic

Cell biology; RNA-seq; Immunoproteins, genes and antigens

Details

  • Operation: Peptide immunogenicity prediction
  • Input: BAM
  • Output: -
  • Software interface: Command-line user interface
  • Language: Python
  • Operating system: Linux; Mac OS X
  • License: Other
  • Cost: Free for non-commercial usage
  • Version name: -
  • Maturity: Mature
  • Credit: The National Health and Medical Research Council of Australia (NHMRC), Australian Centre for HIV and HCV Research, The University of New South Wales (UNSW), NSW Health Early-Mid Career Fellowship, AGRTP.
  • Contact: Fabio Luciani luciani _at_ unsw.edu.au
  • Collection: -

Publications

Rizzetto S, Koppstein DNP, Samir J, Singh M, Reed JH, Cai CH, Lloyd AR, Eltahla AA, Goodnow CC, Luciani F "B-cell receptor reconstruction from single-cell RNA-seq with VDJPuzzle." Bioinformatics. 2018 Aug 15;34(16):2846-2847. https://doi.org/10.1093/bioinformatics/bty203
PMID: 29659703


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