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netSmooth

netSmooth

netSmooth is an R tool for smoothing noisy single-cell scRNA-seq data. The netSmooth algorithm uses a network-diffusion based approach in which it uses priors to analyse the covariance of expression profiles.

Topic

Gene expression; RNA-seq; Sequencing; Transcriptomics

Details

  • Operation: Clustering; Standardisation and normalisation
  • Software interface: Command-line user interface; Library
  • Language: R
  • Operating system: Linux; Mac OS X; Microsoft Windows
  • License: GNU GPL v3
  • Cost: Free
  • Version name: 1.6.0
  • Maturity: Stable
  • Credit: Max Delbrück Center, part of Helmholtz Association.
  • Contact: Altuna Akalin altuna.akalin _at_ mdc-berlin.de | Jonathan Ronen yablee _at_ gmail.com
  • Collection: BioConductor

Publications

Ronen J, Akalin A "netSmooth: Network-smoothing based imputation for single cell RNA-seq." F1000Res. 2018 Jan 3;7:8. https://doi.org/10.12688/f1000research.13511.3
PMID: 29511531
PMCID: PMC5814748.3


Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J1, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M "Orchestrating high-throughput genomic analysis with Bioconductor." Nat Methods. 2015 Feb;12(2):115-21. https://doi.org/10.1038/nmeth.3252
PMID: 25633503
PMCID: PMC4509590


Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JY, Zhang J. "Bioconductor: open software development for computational biology and bioinformatics." Genome Biol. 2004;5(10):R80. Epub 2004 Sep 15. https://doi.org/10.1186/gb-2004-5-10-r80
PMID: 15461798
PMCID: PMC545600


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