2DE-pattern

2DE-pattern catalogs and organizes proteoform profiles derived from two-dimensional electrophoresis (2DE) and shotgun electrospray ionization liquid chromatography–tandem mass spectrometry (ESI-LC-MS/MS) to support systematic proteoform analysis.

Key Features:

• Proteoform Data Repository: Stores proteoform profiles identified from proteomic experiments using 2DE and ESI-LC-MS/MS.
• Isoform-Centric Database Structure: Organizes data into isoform-centric patterns representing proteoforms resolved through two-dimensional electrophoresis.
• Integrated Proteomic Data Sources: Combines protein separation data from two-dimensional electrophoresis with identification results from shotgun ESI-LC-MS/MS.

Scientific Applications:

• Proteoform Cataloging: Compiles proteoform information to characterize diversity of gene products within the human proteome.
• Comparative Proteomics: Enables comparison of proteoform profiles across biological samples or experimental conditions.
• Proteomics Method Development: Provides datasets generated from 2DE and ESI-LC-MS/MS to support proteomic analysis workflows.

Methodology:

2DE-pattern integrates proteoform data obtained through two-dimensional electrophoresis (2DE) separation followed by shotgun electrospray ionization liquid chromatography–tandem mass spectrometry (ESI-LC-MS/MS) identification to organize isoform-centric proteoform profiles.