4See

4See visualizes and integrates 4C (circular chromosome conformation capture) data to analyze chromatin contacts between a chosen bait and distal regulatory elements.

Key Features:

• Flexible Data Plotting: Plots multiple 4C profiles derived from the same bait to compare biological replicates or pooled samples across cell types or experimental conditions.
• Integration Capabilities: Integrates 4C contact profiles with gene tracks, linear epigenomic profiles, and annotated genomic regions.
• Computational Efficiency: Enables rapid exploration of 4C datasets with limited computational resource requirements.

Scientific Applications:

• Chromatin Architecture: Maps chromatin loops and contact patterns to characterize three-dimensional genome organization.
• Promoter-Enhancer Communication: Identifies physical proximity between enhancers and target promoters to investigate transcriptional regulation.
• Comparative 4C Analyses: Compares replicates and conditions to assess differential chromatin interactions across cell types or treatments.

Methodology:

Analyzes data generated by 4C, a 3C derivative that measures contact frequency between a bait locus and other genomic regions.