5NosoAE

5NosoAE performs comparative analyses of nosocomial bacterial strains using antibiograms and whole-genome sequencing to support epidemiological surveillance of antimicrobial resistance.

Key Features:

• Antibiogram-Based Strain Identification: Identifies and compares nosocomial bacterial strains based on antimicrobial resistance profiles (antibiograms).
• Spatiotemporal Distribution Visualization: Visualizes spatiotemporal distribution of strains to track movement and evolution of resistant lineages across locations and time.
• Phylogenetic Analysis: Performs phylogenetic analyses on strains with similar antibiograms to infer evolutionary relationships and trace pathways of resistance genes.
• Comprehensive Data Input: Accepts genome sequence files in FASTA format for Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterococcus faecium, and Staphylococcus aureus.
• Visualization Outputs: Generates antibiogram heatmaps and phylogenetic trees for comparative interpretation of resistance profiles and strain relationships.

Scientific Applications:

• Epidemiological Surveillance: Monitoring and controlling the spread of antimicrobial-resistant strains within healthcare facilities.
• Infection Control Strategies: Informing the development of targeted interventions to mitigate drug-resistant infections.
• Research and Development: Supporting studies into the genetic determinants and evolution of antimicrobial resistance.

Methodology:

Processes whole-genome sequencing (WGS) data using bioinformatics techniques to analyze genomic aspects of antimicrobial resistance and to generate visualizations.