ASRD

ASRD aggregates and standardizes Arabidopsis thaliana small RNA sequencing data to enable comparative analysis of sRNA abundances across genotypes, tissues, and treatment conditions.

Key Features:

• Extensive data repository: Hosts approximately 2.3 billion sRNA reads from around 250 million unique sequences across 2,024 publicly available Arabidopsis sRNA-seq libraries.
• Unified processing pipeline: Reprocesses raw sRNA-seq data from all included libraries using a single pipeline to ensure consistency.
• Standardized normalization: Applies standardized normalization of sRNA abundances to enable direct comparisons of specific sRNAs and aggregate sRNA populations.
• Comparative analysis support: Enables comparison of sRNA abundances across different genotypes, tissues, and treatment conditions.
• Integrated visualization: Provides visualization of sRNA distributions and genomic context via the Integrative Genomics Viewer (IGV).

Scientific Applications:

• Genome stability: Investigating how small RNAs contribute to maintaining genome integrity in Arabidopsis thaliana.
• Disease resistance: Exploring mechanisms by which small RNAs influence resistance to pathogens.
• Developmental regulation: Studying roles of small RNAs in controlling developmental pathways.

Methodology:

Reprocessing of raw sRNA-seq data from included libraries using a unified pipeline with standardized normalization of sRNA abundances; visualization via Integrative Genomics Viewer (IGV).