BAM-matcher

BAM-matcher determines whether two BAM files originate from the same biological source by comparing genotype information at common SNP positions.

Key Features:

• Genotype-Based Matching: Uses genotype information extracted from BAM files to match samples without requiring independently generated high-density SNP data.
• SNP-Position Comparison: Compares genotypes at common single-nucleotide polymorphism (SNP) positions between two BAM files to assess sample identity.
• High-Throughput Compatibility: Performs rapid comparisons suitable for early stages of high-throughput genome sequencing data processing pipelines.
• Sample Mislabeling Detection: Detects sample swaps or mislabeling events within sequencing workflows by genotype concordance analysis.

Scientific Applications:

• Sample Identity Verification: Verifies whether two sequencing datasets derive from the same biological source to detect swaps or mislabeling.
• Matched Tumor–Normal Analysis: Confirms identity of matched tumor and normal samples to support accurate somatic variant calling.

Methodology:

Compares genotypes at common SNP positions extracted from two BAM files to assess whether they originate from the same biological source.