BASILIScan

BASILIScan facilitates high-throughput identification of homologous proteins and evaluation of intrinsic disorder to enable comparative analysis of intrinsically disordered regions (IDRs) across eukaryotic and viral proteomes.

Key Features:

• BLAST-Based Homologue Search: Uses BLAST to identify close homologues of the query protein for comparative sequence analysis.
• Intrinsic Disorder Prediction: Performs simultaneous prediction of intrinsic disorder to map disordered regions alongside sequence homology.
• Homologue Scoring and Selection: Screens, scores, and selects homologues based on predicted structural order to identify more ordered counterparts of the target protein.
• Comparative Analysis of Disorder Conservation: Conducts parallel comparative analysis to examine conservation of extended disordered regions across multiple sequences.

Scientific Applications:

• Recombinant Protein Expression: Identifies intrinsically stable homologues with poorly conserved disordered regions to improve chances of successful recombinant expression.
• Crystallization and Downstream Applications: Pinpoints structured homologues that are more compatible with crystallization and other structural or functional assays.
• Biological Insights: Reveals that portions of the human proteome have homologous sequences in related species with superior intrinsic structural order, informing evolutionary and protein-engineering studies.

Methodology:

Performs a BLAST-based homologue search, simultaneous intrinsic disorder prediction, parallel comparative analysis of disorder conservation, and scoring/selection of homologues based on predicted structural order.