BMCeasy

BMCeasy computes benchmark concentrations (BMCs) and their statistical uncertainties from concentration–response data to quantify effects in cytotoxicity tests and functional in vitro assays.

Key Features:

• Benchmark concentration (BMC) calculation and uncertainty estimation: Determines absolute BMCs and quantifies their statistical uncertainties.
• Concentration–response modeling for cytotoxicity and functional in vitro assays: Models concentration–response relationships specific to cytotoxicity tests and functional in vitro endpoints.
• Simultaneous multi-endpoint analysis: Performs concurrent analysis of multiple endpoints such as cell viability and functional test outcomes.
• Benchmark Response (BMR) flexibility: Allows specification of BMR values (e.g., 10%, 25%, 50% deviations from control) to define effect levels.
• Confidence interval computation: Provides confidence intervals for BMC estimates to express statistical uncertainty.

Scientific Applications:

• Toxicological hazard and risk assessment: Enables determination of concentration thresholds used in chemical safety evaluations and risk profiling.
• Regulatory submission support: Produces BMCs and associated uncertainty estimates applicable to regulatory submissions and frameworks such as EU-ToxRisk.
• In vitro assay analysis: Supports analysis of concentration–response effects in cytotoxicity tests and functional in vitro assays.

Methodology:

Curve fitting to concentration–response data is used to identify concentrations at which predefined benchmark responses occur and to derive BMCs with confidence intervals.