BRAPeS

BRAPeS reconstructs B cell receptor (BCR) sequences from short-read paired-end single-cell RNA-sequencing data to enable analysis of B cell state and receptor specificity.

Key Features:

• High accuracy and success rate: Reconstructs BCR sequences with high accuracy from very short reads, including read lengths as short as 25 base pairs.
• Short-read paired-end optimization: Operates on paired-end single-cell RNA-sequencing data and is optimized to recover full-length BCR sequences from short reads.
• Cost-effectiveness and scalability: Uses short-read sequencing to reduce per-cell sequencing cost and enable analysis of larger numbers of cells in single-cell experiments.

Scientific Applications:

• Single-cell analysis: Enables characterization of individual B cells by linking cellular state with reconstructed receptor sequences at the single-cell level.
• Immunological research: Facilitates studies of BCR diversity and clonality relevant to immune responses, vaccine development, and autoimmune disease research.

Methodology:

BRAPeS leverages paired-end short-read single-cell RNA-sequencing data to reconstruct full-length BCR sequences, with methods optimized for short reads down to 25 base pairs.