BiasDB

BiasDB catalogs literature-reported biased G protein-coupled receptor (GPCR) ligands and their signaling profiles for comparative analysis.

Key Features:

• Comprehensive data collection: Contains 615 documented cases of signaling bias covering 481 individual ligands across 61 receptors, with entries aggregated from the literature.
• Detailed ligand annotations: Records include chemical structure, target receptor, type of signaling bias, assay categories used for determining bias, reference ligands, and literature sources.
• Manual curation and analysis: Entries are manually curated and the assembled dataset is analyzed to identify trends and patterns in biased GPCR signaling.

Scientific Applications:

• Medicinal chemistry: Supports design and optimization of biased ligands by providing annotated examples of signaling bias and reference compounds.
• Pharmacology: Enables comparative analysis of GPCR signaling pathways and the functional selectivity of ligands across assay categories.
• Research and hypothesis generation: Serves as a compiled reference for exploring biased GPCR signaling, planning experiments, and deriving structure–activity relationships.

Methodology:

Entries were compiled through rigorous manual curation of published literature reporting biased GPCR ligands and associated assay annotations, and the dataset was analyzed to characterize bias patterns.