BioGRID Open Repository of CRISPR Screens (ORCS)

BioGRID Open Repository of CRISPR Screens (ORCS) aggregates and curates genome-wide CRISPR/Cas9 screen datasets to provide standardized gene-phenotype and genetic interaction data for functional genomics.

Key Features:

• Extensive Data Coverage: Contains over 895 CRISPR screens across three model organism species and 629 cell lines, including more than 500 genome-wide CRISPR/Cas9 screens in human and mouse cell lines.
• Genome-wide CRISPR/Cas9 Focus: Concentrates on genome-scale loss-of-function and perturbation screens to report phenotypic outcomes of gene disruption.
• Gene-phenotype and Genetic Interaction Data: Provides curated gene-phenotype associations and gene-gene relationship data derived from CRISPR screens.
• Standardized Formats: Data are available in a variety of standardized file formats for downstream computational analysis.
• Curated Annotation with Controlled Vocabularies: Entries are manually annotated and curated using controlled vocabularies for experimental detection methods.
• Integration with BioGRID Corpus: Linked to BioGRID, which as of September 2018 (BioGRID build 3.4.164) contained 1,598,688 manually annotated biological interactions from 55,809 publications across 71 species.

Scientific Applications:

• Functional Genomics: Assigns gene function by linking gene disruption to cellular phenotypes from genome-wide screens.
• Drug Target Identification: Supports identification and prioritization of therapeutic targets through phenotype- and interaction-based evidence.
• Genetic Interaction Mapping: Enables discovery and analysis of gene-gene relationships and synthetic interactions from CRISPR screen data.
• Comparative Analysis Across Cell Lines and Species: Facilitates cross-cell-line and cross-species comparisons of CRISPR screen results to assess context-specific gene functions.

Methodology:

Manual annotation of screen results and biological interactions, curation using controlled vocabularies for experimental detection methods, and distribution of data in standardized file formats.