CFQuant-automatic

CFQuant-automatic quantifies luminescent and fluorescent halos around cell colonies in high-throughput images to provide quantitative measurements for colony luminescence assays.

Key Features:

• Automatic Identification and Quantification: Automatically identifies cell colonies and their associated luminescent or fluorescent halos in images despite biological noise.
• Shape-Based Analysis: Leverages the expected shape of luminescence halos to improve detection and measurement accuracy.
• Quantitative Data Output: Produces detailed quantitative measurements of features related to colonies and their luminescent halos.
• High-throughput Image Analysis and Validation: Performs high-throughput image analysis and was validated by correlation with known protein expression levels (R = 0.85).

Scientific Applications:

• Colony luminescence assays: Quantifies luminescence and fluorescence levels around colonies for reporter-based assays in microbiological research.
• Comparative trait analysis: Enables comparison of luminescence-derived traits across different strains or treatments.

Methodology:

High-throughput image analysis that identifies colonies and luminescent/fluorescent halos using shape-based criteria, with validation by correlation to protein expression levels (R = 0.85).