CIRCexplorer2

CIRCexplorer2 annotates circular RNAs (circRNAs) and their alternative back-splicing and splicing isoforms genome-wide from RNA sequencing datasets.

Key Features:

• Genome-Wide Annotation: Performs genome-wide identification and annotation of circRNAs across species from RNA sequencing datasets.
• Alternative Back-Splicing and Splicing Events: Detects multiple circRNA isoforms generated from a single gene locus through alternative back-splicing and splicing events.
• Systematic Computational Pipeline: Implements a stepwise computational scheme to analyze RNA sequencing data for circRNA discovery and characterization.
• Functional Characterization: Produces detailed annotations of circRNA isoforms and their splicing variants to support downstream functional analyses.

Scientific Applications:

• Transcriptome Diversity Studies: Identifies and annotates diverse circRNA isoforms to assess transcriptome complexity and alternative splicing patterns.
• Functional Genomics Research: Provides annotated circRNA isoforms and splicing variants for studies of circRNA roles in biological processes and disease.
• Comparative Genomics: Enables comparison of circRNA profiles across species to inform evolutionary and comparative analyses.

Methodology:

Computational pipeline analyzes RNA sequencing data to detect back-splicing events and annotate circRNA isoforms and splicing variants.