CRISPR Explorer

CRISPR Explorer designs guide RNAs (gRNAs) for CRISPR-Cas9 genome editing and ranks candidate gRNAs by predicted specificity and efficiency.

Key Features:

• Empirical Scoring Schemes: Incorporates published empirical scoring schemes for gRNA specificity and efficiency.
• Off-target Analysis: Evaluates potential off-target sites for candidate gRNAs to assess specificity.
• Batch Design Mode: Enables batch specification of gRNA selection parameters and accepts multiple input formats.
• Real-time Result Generation: Produces gRNA design results in real time.

Scientific Applications:

• Targeted Genome Editing: Design of gRNAs for precise targeted gene modifications using CRISPR-Cas9.
• Functional Genomics: Selection of gRNAs for loss- or gain-of-function experiments in functional genomics studies.
• Therapeutic Development: Support for designing gRNAs in therapeutic development involving CRISPR-Cas9.

Methodology:

Integrates empirical data on gRNA specificity and efficiency into scoring algorithms.