Cas-analyzer

Cas-analyzer analyzes genome editing outcomes from Next Generation Sequencing (NGS) data to quantify mutation frequencies and assess editing efficiency and specificity.

Key Features:

• Client-Side Processing: Implemented in JavaScript to execute entirely within a client-side web browser and perform on-the-fly processing of NGS datasets.
• NGS-based Mutation Quantification: Computes mutation frequencies from NGS reads to enable measurement of genome editing outcomes.
• Support for Programmable Nucleases: Accommodates various programmable nucleases, including CRISPR-Cas9 and both single and paired nuclease configurations.

Scientific Applications:

• Mutation Frequency Quantification: Quantifying mutation frequencies induced by CRISPR-Cas9 and other programmable nucleases using NGS data.
• Assessment of Editing Efficiency and Specificity: Assessing efficiency and specificity of genetic modifications from NGS datasets.
• Genetics, Biotechnology, and Therapeutic Research: Supporting studies that require precise evaluation of genome editing outcomes for genetic research, biotechnology, and therapeutic development.

Methodology:

Implemented in JavaScript to perform client-side computation and on-the-fly processing of NGS datasets within a web browser, avoiding remote data uploads.