CellTag

CellTag enables genetic barcode-based sample multiplexing and longitudinal lineage tracing in single-cell genomics.

Key Features:

• Genetic Barcode-Based Multiplexing: Employs predefined lentiviral barcodes that are heritable and enable tagging, pooling, and tracking of cell populations.
• High-Throughput Compatibility: Optimized for high-throughput single-cell assays and addresses experimental design, sample multiplexing, batch effect removal, and data interpretation.
• Versatility Across Cell Types: Demonstrated applicability across diverse cell types, allowing use in multiple biological contexts.
• Long-Term Tracking Capabilities: Enables longitudinal tracking of cellular processes such as engraftment and differentiation in vivo.

Scientific Applications:

• Lineage Tracing: Uses unique barcodes to trace the lineage and fate of individual cells over time.
• Single-Cell Transcriptome Sequencing (scRNA-seq): Facilitates multiplexing of samples for scRNA-seq to increase throughput and reduce costs in transcriptomic studies.
• In Vivo Studies: Supports tracking of cell behavior and fate within living organisms, including engraftment and differentiation dynamics.

Methodology:

Integration of lentiviral barcodes into target-cell genomes and detection of these stable, heritable barcodes by sequencing after pooling tagged cells for multiplexed analysis.