ColiCoords

ColiCoords transforms Cartesian coordinates from transmission light, fluorescence images, and single-molecule localization microscopy (SMLM) data into a cellular coordinate system for rod-like bacterial cells to enable single-cell structuring and population-level quantitative analysis.

Key Features:

• Single-Cell Level Structuring: Organizes microscopy data at the single-cell level using a coordinate system tailored for rod-like bacterial cells.
• Coordinate Transformation: Converts Cartesian coordinates from transmission light, fluorescence images, and SMLM into cellular coordinates.
• Multi-Cell Data Integration: Aligns and combines cellular coordinates from multiple cells to increase statistical power across fluorescence microscopy datasets.
• Supported Data Types: Explicit handling of transmission light images, fluorescence images, and single-molecule localization microscopy (SMLM) data.

Scientific Applications:

• Single-Molecule Localization Analysis: Analysis of protein machineries and cellular processes in rod-like bacteria using SMLM and fluorescence datasets.
• Population-Level Quantitative Comparison: Aggregation of cellular coordinates across cells for statistical assessment of spatial distributions and localization patterns.

Methodology:

Transforms Cartesian coordinates from transmission light, fluorescence images, and SMLM into a rod-like cellular coordinate system, structures data per cell, and aggregates cellular coordinates across cells for population-level analysis.