Complex Portal

Complex Portal compiles and curates detailed information on macromolecular complexes composed of proteins, nucleic acids, and small molecules to support analysis of complex composition, topology, and biological function.

Key Features:

• Manually Curated Complex Database: Provides expert-curated macromolecular complex entries integrating information from ChEMBL, MatrixDB, PDBe, Reactome, and UniProtKB.
• Comprehensive Complex Annotations: Annotates participating molecules, stoichiometry, topology, structural assembly, functional properties, and Gene Ontology (GO) terms.
• Evidence Code Ontology Integration: Uses Evidence Code Ontology to indicate whether complex annotations are supported by experimental evidence or inferred from homology or orthology.
• Standardized Nomenclature: Assigns systematic names, recommended names, and synonyms for consistent identification of macromolecular complexes.
• Multi-Species Complex Coverage: Contains curated macromolecular complex information across 20 species.
• Standardized Data Formats: Provides downloadable complex datasets in PSI-XML, MI-JSON, and ComplexTAB formats.
• Identifier-Based Querying: Supports searches using UniProt, ChEBI, and Gene Ontology identifiers as well as protein, gene, and complex names.
• Saccharomyces cerevisiae Complexome: Includes a curated draft complexome dataset for Saccharomyces cerevisiae.

Scientific Applications:

• Macromolecular Complex Analysis: Investigates the composition, structure, and function of protein complexes and multi-component molecular assemblies.
• Systems Biology Research: Explores biological pathways and interaction networks through curated complex datasets.
• Comparative Molecular Studies: Examines conservation and variation of macromolecular complexes across multiple species.

Methodology:

Complex Portal applies manual expert curation of macromolecular complexes using integrated data from ChEMBL, MatrixDB, PDBe, Reactome, and UniProtKB, annotates complexes with standardized nomenclature and Gene Ontology terms, and assigns evidence using Evidence Code Ontology.