Human Dephosphorylation Database (DEPOD)

Human Dephosphorylation Database (DEPOD) curates a comprehensive, manually curated repository of human phosphatases, their protein and non-protein substrates, specific dephosphorylation sites, and linked annotations to support analysis of phosphatase–kinase signaling and disease associations.

Key Features:

• Curated Data: Contains information on 254 human phosphatases, 336 protein substrates, 83 non-protein substrates, and 1215 manually curated phosphatase–substrate relationships.
• Dephosphorylation Site Information: Provides specific dephosphorylation site annotations for substrates.
• Pathway Integration: Links phosphatases to their associated signaling pathways.
• Extensive Database Linkages: Integrates annotations from 69 open-access databases, including disease associations, phosphorylating kinases, protein interactions, and genome browser links.
• Visualization Tools: Provides visualization of protein interactions, protein structures, phosphorylation networks, evolutionary conservation, and short linear motif identification.

Scientific Applications:

• Phosphatase–Kinase Network Analysis: Supports analysis of phosphatase–kinase networks and regulatory mechanisms in cellular signaling.
• Disease Association Studies: Enables investigation of disease associations linked to dysregulated phosphorylation and identification of candidate therapeutic targets.

Methodology:

Manual curation of literature-derived phosphatase–substrate relationships and integration of annotations from 69 open-access databases, with provision of visualization for interactions, structures, phosphorylation networks, evolutionary conservation, and short linear motif identification.