DEUS

DEUS analyzes raw sequencing reads to quantify expression levels of small RNAs without reference genome alignment, reducing ambiguous-mapping errors and accurately representing small RNA populations.

Key Features:

• Mapping-Free Expression Profiling: DEUS assesses expression directly from raw sequence data without the need for reference genome alignment.
• Differential Expression Analysis: Identifies sequences with significant expression changes across conditions using individual sequence counts.
• Annotation and Clustering: Annotates identified sequences against user-defined databases and clusters them by sequence similarity to link to known biological functions.

Scientific Applications:

• Gene Regulation Studies: Profiles small RNA regulation of gene expression under different conditions.
• Disease Research: Investigates small RNAs as candidate biomarkers or therapeutic targets in diseases such as cancer.
• Developmental Biology: Characterizes roles of small RNAs in developmental processes and organismal growth.

Methodology:

Sequence-based expression profiling from raw reads without genome alignment; counting individual sequences for differential expression; annotation against user-defined databases and clustering by sequence similarity.