DNA-BOT

DNA-BOT automates low-cost, multi-part DNA assembly to enable construction of large libraries of DNA constructs for synthetic and molecular biology.

Key Features:

• Low-cost automation: Performs automated DNA assembly with consumable costs reported between $1.50 and $5.50 per construct.
• High accuracy and throughput: Demonstrated simultaneous assembly of 88 constructs composed of 10 genetic parts, indicating high accuracy for complex designs.
• Integration with standardized assembly and liquid-handling hardware: Implements the BASIC standardized assembly method on OpenTrons liquid-handling hardware to execute automated builds.

Scientific Applications:

• Exploration of genetic design space: Enables construction of extensive libraries of DNA constructs for systematic exploration of genetic design parameters.
• Evaluation of genetic components in operon contexts: Facilitates evaluation of promoters, ribosome binding sites, and gene order within a three-gene operon design space.

Methodology:

Uses the BASIC standardized assembly method executed on OpenTrons liquid-handling hardware to automate multi-part DNA assembly.