DetSpace

DetSpace enables design of genetic circuits that convert target compounds via enzymatic reactions into detectable molecules and integrate transcription factor–based biosensors to dynamically regulate metabolic pathways for optimizing bio-based chemical production, including titers, rates, and yields.

Key Features:

• Integrated Toolset: Provides a suite for designing genetic circuits that couple enzymatic conversion of target compounds to detectable molecules and recognition by transcription factors.
• Automated Design Process: Systematically generates biological circuit designs that are responsive to specified concentrations of target compounds.
• Dynamic Pathway Regulation: Incorporates biosensors into metabolic pathways to enable dynamic control aimed at improving titers, rates, and yields in bio-based chemical production.

Scientific Applications:

• Genetic Circuit Design: Design of genetic circuits that dynamically regulate metabolic pathways to improve production of bio-based chemicals.
• Strain Optimization: Automated screening and dynamic pathway regulation for optimizing microbial strains for scale-up and higher production metrics.
• Bioremediation and Diagnostics: Tailoring biosensing routes for environmental bioremediation applications and medical diagnostic sensing.
• Drug Delivery Systems: Designing biosensor-coupled metabolic control strategies relevant to controlled drug delivery applications.

Methodology:

Conversion of target molecules into detectable forms via enzymatic reactions and design/selection of biological circuits that couple transcription factor recognition to dynamic regulation of metabolic pathways.