Diat.barcode

Diat.barcode provides a curated rbcL reference library for DNA-barcoding of diatoms (Bacillariophyta) to enable species-level taxonomic assignment and ecological assessment from High-Throughput-Sequencing data.

Key Features:

• rbcL reference sequences: A curated collection of rbcL (chloroplast) sequences targeted for species-level identification of diatoms.
• Data aggregation: Integrates sequence data sourced from the NCBI nucleotide database and unpublished sequencing from culture collections.
• High-Throughput-Sequencing support: Intended for DNA-barcoding analyses of environmental samples generated by High-Throughput-Sequencing technology.
• Temporal curation: Library maintenance initiated in 2012 with a focused collaborative curation effort on rbcL since 2017.
• Taxonomic and ecological scope: Covers diatoms (Bacillariophyta) from both oceanic and freshwater ecosystems.

Scientific Applications:

• Taxonomic assignment: Species-level identification of diatom sequences using the rbcL marker.
• Ecological assessment: Use of diatom barcodes to assess aquatic environmental health and productivity in oceanic and freshwater systems.
• Environmental sample analysis: Support for analysis of numerous environmental samples collected for DNA-barcoding with High-Throughput-Sequencing.

Methodology:

Aggregates sequences from the NCBI nucleotide database and unpublished culture collection sequencing, with curation focused on the rbcL chloroplast marker for DNA-barcoding of diatoms and intended use with High-Throughput-Sequencing data.