EPIC-TABSAT

EPIC-TABSAT analyzes targeted bisulfite sequencing (TBS) data and integrates results with array-based DNA methylation datasets to support epigenetic profiling and validation studies.

Key Features:

• Targeted Bisulfite Sequencing Analysis: Processes TBS datasets to compute DNA methylation levels across targeted genomic regions.
• Integration with Array-Based Methylation Data: Combines targeted sequencing results with chip-based DNA methylation datasets for comparative analysis.
• Quality Metric Calculation: Computes sequencing quality metrics as part of TBS data analysis.
• Methylation Calling: Performs methylation state determination from bisulfite sequencing reads.
• Parallel Processing of Targets: Analyzes multiple sequencing files and genomic targets concurrently.
• Epiallele Computation: Calculates target-specific epialleles for detailed methylation pattern analysis.

Scientific Applications:

• Epigenomic Profiling: Investigates DNA methylation patterns using targeted bisulfite sequencing data.
• Biomarker Validation: Validates epigenetic biomarkers identified in genome-wide methylation studies.
• Clinical Epigenetics Research: Supports studies of methylation-based diagnostic and prognostic biomarkers.

Methodology:

EPIC-TABSAT calculates sequencing quality metrics, performs methylation calling from targeted bisulfite sequencing reads, computes target-specific epialleles, and integrates TBS results with array-based methylation datasets.