GUV-AP

GUV-AP performs quantitative image analysis of Giant Unilamellar Vesicles (GUVs) by extracting fluorescence-based measurements of membrane dye incorporation and protein binding from microscopy images.

Key Features:

• Automated image analysis: Automated recognition and processing of digital fluorescence microscopy images of GUVs for batch analysis.
• Fluorescence imaging analysis: Extraction and quantification of fluorescence signals to characterize membrane dye distribution and intensity.
• Quantitative protein binding assessment: Fluorescence intensity–based method to quantify protein binding to GUV membranes.
• FIJI/ImageJ macro implementation: Implemented as a macro for FIJI/ImageJ to apply consistent image-processing criteria across samples and datasets.

Scientific Applications:

• Membrane biophysics: Quantitative characterization of membrane composition and dynamics in GUV model systems.
• Protein–membrane interaction studies: Measurement of protein binding affinity and distribution on synthetic membranes.
• Cell biology and biochemical modeling: Investigation of processes related to membrane signaling, transport, and membrane fusion using GUVs as model membranes.

Methodology:

Automated image-processing routines implemented as a FIJI/ImageJ macro perform recognition and processing of digital fluorescence microscopy images and extract fluorescence intensity measurements; the macro applies consistent analysis criteria across datasets for quantitative assessment of membrane dye incorporation and protein binding.