GUcal

GUcal calculates glucose unit (GU) values from capillary electrophoresis (CE) electropherograms to support structural elucidation and tentative assignment of N-glycans.

Key Features:

• Automated GU Value Calculation: Calculates glucose unit (GU) values automatically from CE electropherograms to enable high-throughput GU determination.
• Tentative Structure Suggestion: Suggests potential N-glycan structures by querying an internal database with calculated GU values.
• Expanded Database Integration: Integrates publicly available CE data from the GlycoStore collection and in-house measured GU values into its internal database to broaden reference coverage.
• Internal/Bracketing Standard Approach: Employs a bracketing standard calibration method to ensure precise GU calibration and reliable quantification of glycan components in complex mixtures.

Scientific Applications:

• High-throughput N-glycan profiling: Supports large-scale profiling of N-glycans by providing automated GU determination from CE data.
• First-line structural elucidation: Provides rapid, first-line tentative assignment of N-glycan structures to guide subsequent validation with complementary techniques.
• Calibration and quantitative analysis: Enables calibration and quantitative comparison of glycan mixtures using bracketing standards and reference GU values.

Methodology:

Integration of CE data into an internal database, calibration using bracketing standards, and automated calculation of GU values with database-driven tentative structure suggestion.