GlycanAnalyzer

GlycanAnalyzer interprets N-glycan profiles from exoglycosidase digestion data to assign detailed glycan structures using UPLC/HPLC and MS1 peak-shift patterns.

Key Features:

• Automated Pattern Matching: Employs pattern-matching algorithms to interpret signature peak shifts in UPLC/HPLC and MS1 data resulting from exoglycosidase digestion, where specific monosaccharide removals cause characteristic retention time and mass changes.
• High Precision Structure Assignment: Uses detailed peak-shift data to assign N-glycan structures with precision including monosaccharide position and isomeric linkage, achieving 100% assignment accuracy in tests with a monoclonal antibody and four glycan standards versus up to 82% for other software.
• Integration with Standard Databases: Integrates observed retention times and mass data with standardized reference values from established databases to support automated structure assignments.

Scientific Applications:

• N-glycan Structural Characterization: Automates interpretation of exoglycosidase digestion data for detailed structural elucidation of N-glycans.
• Protein Glycosylation Analysis: Supports studies of protein glycosylation patterns relevant to biological processes and disease mechanisms.

Methodology:

Processes UPLC/HPLC coupled MS1 data from exoglycosidase digestions, detects retention time and mass peak shifts, applies pattern-matching algorithms, and compares observed shifts to reference retention times and masses from databases to assign glycan structures.