Green listed

Green listed enables rapid design of custom CRISPR screens targeting specified gene sets to support functional genomics investigations using CRISPR/Cas9.

Key Features:

• Customizable Targeting: Accepts user-defined gene sets and allows selection of targets based on criteria such as differential expression or pathway membership.
• CRISPR/Cas9-based Screen Design: Leverages the bacterial CRISPR/Cas9 system to generate designs for screens that target specified genes.
• Secondary Screen Generation: Supports generation of targeted secondary screens to refine and follow up on findings from large-scale CRISPR screens.

Scientific Applications:

• Differential-expression-targeted screens: Design screens that focus on genes differentially expressed under specific conditions to investigate regulatory mechanisms.
• Pathway-focused screens: Target genes associated with particular biological pathways or functions for pathway analysis and functional annotation.
• Secondary/validation screens: Create targeted follow-up screens after large-scale screening to validate and explore initial hits in greater detail.

Methodology:

Users input gene sets of interest, and the system utilizes the CRISPR/Cas9 system to design screens that target those genes.