ISIMA

ISIMA quantifies cell confluence from phase-contrast micrographs to provide reproducible, quantitative measurements for validating proliferation and viability assays.

Key Features:

• Segmentation pipeline: ilastik is used for pixel-classification segmentation of phase-contrast micrographs.
• Area measurement: ImageJ is used to measure segmented area and compute percent confluence.
• Monolayer analysis: Tailored for analysis of cell monolayers, including Hep G2 cell cultures.
• Correlation with MTT assay: Confluence measurements have been tested for correlation with the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.

Scientific Applications:

• Confluence quantification in Hep G2 cultures: Quantifies percent confluence from phase-contrast micrographs of Hep G2 monolayers.
• Validation of proliferation and viability assays: Provides quantitative confirmation for assays such as the MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay.
• Assessment of assay interference: Aids interpretation of experimental outcomes by identifying confluence-related interference from test substances.
• Use in cell biology, toxicology, and pharmacology: Supports reproducible confluence measurements for studies in these fields.

Methodology:

ilastik pixel-classification segmentation of phase-contrast micrographs followed by ImageJ area measurement to compute percent confluence.