ImSpectR

ImSpectR quantifies immune repertoire diversity by scoring deviations in CDR3 length distributions derived from spectratype, bulk repertoire sequencing, and single-cell RNA sequencing to provide objective measures for quantitative immunological analyses.

Key Features:

• Unified Approach: Analyzes multiple data types including spectratype, repertoire sequencing, and single-cell RNA sequencing within a unified framework.
• Quantitative Analysis: Scores deviations from expected length distributions of rearranged T- and B-cell receptors (CDR3) and integrates different deviation types into a single numerical measure for comparative analyses.
• Reduction of Subjectivity: Replaces visual inspection with objective, standardized scoring to reduce subjective bias in diversity assessment.

Scientific Applications:

• Clinical Diagnostics: Assisting diagnosis of primary immune deficiencies by providing quantitative measures of repertoire diversity.
• Prognostic Evaluations: Informing disease prognosis through analysis of immune repertoire variability.
• Therapeutic Monitoring: Monitoring patient responses to therapies via quantitative changes in repertoire diversity.

Methodology:

Quantifies CDR3 length distributions from T-cell receptor (TCR) sequencing and spectratype data, scores deviations from expected length distributions of rearranged T- and B-cell receptors, integrates deviation types into a single measure, and has been demonstrated on human TCR sequencing data from the ImmunoSeq platform and on CDR3 data from CMV+ PBMCs (Suessmuth et al.).