LM-GlycomeAtlas

LM-GlycomeAtlas visualizes and maps glycomic profiles generated by lectin microarray analyses using 45 distinct lectins to characterize site- and tissue-specific glycosylation patterns in formalin-fixed paraffin-embedded tissue sections.

Key Features:

• Tissue Glycome Mapping: Employs laser microdissection-assisted lectin microarray (LMA) to enable high-throughput, reproducible glycomic profiling of formalin-fixed paraffin-embedded tissue sections.
• Lectin Coverage: Analyzes binding data from 45 distinct lectins to map glycan structures across different cell types, sites, and tissues.
• Data Comparison: Enables comparative analysis between LMA-based glycomic data and mass spectrometry-based glycomic profiles such as those in GlycomeAtlas.
• Extensive Dataset: Includes the initial 234 glycomic profiling datasets from nine tissue sections of two 8-week-old male C57BL/6J mice and an expanded collection of 451 glycomic profiles and 42 high-resolution histological images from 14 tissues of normal and diseased mice.
• Enhanced Spatial Information: Integrates multiple datasets with spatial context, including staining images using various lectins on the array alongside histological images.
• Integration with Glycoproteomic Resources: Supports interoperability with other resources for glycoproteomic data and protein glycosylation machinery analysis.

Scientific Applications:

• Protein Glycosylation Analysis: Characterizes biological roles and disease-associated alterations of protein glycosylation within tissue samples.
• Site- and Tissue-specific Glycosylation Mapping: Identifies and compares glycosylation patterns across cell types, anatomical sites, and tissues using lectin binding profiles and mass spectrometry comparisons.
• Glyco-pathological Studies and Biomarker Research: Supports investigations of tissue-specific glycan alterations relevant to pathology and the development of diagnostic or therapeutic strategies.
• Glycoproteomics Integration: Facilitates combined analyses with proteomics and glycoproteomic datasets to study protein glycosylation machinery and glycan–protein interactions.

Methodology:

Processes lectin microarray data produced by laser microdissection-assisted lectin microarray (LMA) on formalin-fixed paraffin-embedded tissues using 45 lectins and supports comparison with mass spectrometry-based glycomic profiles (e.g., GlycomeAtlas).