LuxR

LuxR is a master quorum-sensing transcription factor in Vibrio harveyi that regulates over 100 genes in response to changes in population density. The study shows that LuxR activates gene expression by competing with and displacing H-NS, a global transcriptional repressor that forms filaments and bridges on DNA to repress gene expression. LuxR and H-NS co-regulate and co-occupy 28 promoters, driving the expression of 63 genes. As autoinducer concentration increases, LuxR accumulates at these promoters while H-NS disperses. LuxR is sufficient to evict H-NS from promoter DNA in vitro, which depends on LuxR's DNA binding activity. The proposed model suggests that LuxR acts as a counter-silencer at H-NS-repressed quorum sensing loci by disrupting H-NS nucleoprotein complexes that block transcription.

Topic

Transcription factors and regulatory sites;RNA-Seq;ChIP-seq;PCR experiment;ChIP-on-chip

Detail

  • Operation: Peak calling;Operon prediction;Promoter prediction;PCR primer design;Formatting

  • Software interface: Command-line interface

  • Language: Perl

  • License: Not stated

  • Cost: Free of charge

  • Version name: -

  • Credit: The National Institutes of Health (NIH) and the National Science Foundation (NSF).

  • Input: -

  • Output: -

  • Contact: Julia C van Kessel jcvk@indiana.edu

  • Collection: -

  • Maturity: -

Publications

  • Global H-NS counter-silencing by LuxR activates quorum sensing gene expression.
  • Chaparian RR, et al. Global H-NS counter-silencing by LuxR activates quorum sensing gene expression. Global H-NS counter-silencing by LuxR activates quorum sensing gene expression. 2020; 48:171-183. doi: 10.1093/nar/gkz1089
  • https://doi.org/10.1093/NAR/GKZ1089
  • PMID: 31745565
  • PMC: PMC7145609

Download and documentation

< Back to DB search