M-TRACK

M-TRACK analyzes live-cell multiplex imaging to uncover dynamic phenotypic transitions and temporal trajectories in single cells.

Key Features:

• Endogenous fluorescent labeling: Integration of endogenous fluorescent labeling techniques that minimize perturbation to cellular physiology.
• Live-cell multiplex imaging: Advanced live-cell imaging that captures high-dimensional morphological and texture features over time.
• Temporal trajectory extraction: Extraction and analysis of live-cell trajectories to reveal dynamic phenotypic transitions not observable in snapshot data.
• Addresses snapshot limitations: Overcomes the inability of fixed cell-based methods to capture temporal dynamics in single-cell studies.
• Long-term multiplex fluorescence handling: Tackles technical challenges associated with fluorescence-based live-cell imaging for long-term, multiplex studies.

Scientific Applications:

• EMT in A549 VIM-RFP reporter line: Analysis of epithelial-to-mesenchymal transition using an A549 VIM-RFP EMT reporter line to reveal parallel EMT trajectories masked in static datasets.
• Cellular heterogeneity and phenotypic transitions: Extraction of dynamical information from multiplex live-cell imaging to study cell-cell heterogeneity and phenotypic state changes.

Methodology:

Extraction and analysis of live-cell imaging trajectories and time-series of high-dimensional morphological and texture features derived from endogenous fluorescent labels.