MAC-INMV-SSR

MAC-INMV-SSR performs genotyping of Mycobacterium avium complex (MAC) strains, with emphasis on Mycobacterium avium subsp. paratuberculosis (Map), by standardizing and indexing MLVA (targeting mycobacterial interspersed repetitive units (MIRUs) and variable number tandem repeats (VNTRs)) and MLSSR signatures to support epidemiological surveillance and comparative analyses.

Key Features:

• Genotyping Methodology: Implements multi-locus variable number tandem repeat analysis (MLVA) targeting MIRUs and VNTRs using eight specific markers for high discrimination among strains.
• Integration with MLSSR Sequencing: Supports multi-locus short-sequence-repeat (MLSSR) sequencing and the combined use of MLVA and MLSSR to increase genotyping resolution and reliability.
• Standardization and Data Sharing: Indexes and enables sharing of existing profiles and newly defined genotypes to standardize practices and ensure consistency of genotype interpretation across laboratories.

Scientific Applications:

• Epidemiological surveillance and outbreak investigation: Enables tracking of MAC and Map strain distribution and identification of outbreak-related isolates through MLVA/MLSSR profile comparison.
• Population genetics and evolutionary analysis: Supports assessment of genetic diversity and population structure within MAC and Map using VNTR/MIRU and MLSSR data.
• Comparative genotyping and standardization studies: Facilitates aggregation and comparison of genotype data to harmonize subtype definitions and inform targeted intervention strategies.

Methodology:

Uses MLVA targeting MIRUs and VNTRs with eight specific markers; applies MLSSR sequencing; and permits combined analysis of MLVA and MLSSR profiles.