MIBiG 2.0

MIBiG 2.0 provides a curated repository of biosynthetic gene clusters (BGCs) of known function to support genome mining and the characterization of specialized metabolite biosynthesis.

Key Features:

• Curated BGC collection: Contains 851 newly added BGCs and extensive manual curation to improve annotation quality across entries.
• Schema refinement: Uses a redesigned data schema aligned with JSON Schema Draft V7 to standardize and validate BGC annotations.
• REST API layer: Provides a REST API layer for programmatic access to BGC records and integration into computational workflows.
• Minimum Information standard: Adheres to the Minimum Information about a Biosynthetic Gene Cluster (MIBiG) Data Standard to ensure completeness and required metadata for curated BGCs.

Scientific Applications:

• Drug discovery: Enables identification and comparison of known BGCs to support discovery and characterization of specialized metabolite biosynthetic pathways relevant to drug discovery.
• Genome mining and pathway characterization: Supports genome mining by providing reference BGCs to interpret the function and novelty of candidate clusters.
• Microbial ecology and microbiome research: Facilitates studies of microbial ecology and microbiome-associated specialized metabolite production by providing annotated BGCs of known function.

Methodology:

Rigorous manual data curation, schema redesign aligning annotations to JSON Schema Draft V7, and implementation of a REST API layer.