MethBase

MethBase provides a reference methylome database constructed from publicly available whole-genome bisulfite sequencing (BS-seq) datasets for comparative DNA methylation analysis across organisms.

Key Features:

• Reference methylomes: Annotated methylome assemblies derived from public BS-seq datasets covering multiple organisms.
• Single-base resolution: Leverages whole-genome bisulfite sequencing (BS-seq) data to represent DNA methylation at single-base resolution.
• Feature extraction: Facilitates extraction and comparison of methylation features across methylomes.
• Annotation: Provides annotated methylomes to support identification of methylation patterns and variation.
• Integration with MethPipe: Operates as part of a suite that includes MethPipe low- and high-level analysis pipelines.

Scientific Applications:

• Comparative methylome analysis: Compare DNA methylation profiles across organisms and experimental conditions.
• Methylation pattern discovery: Identify patterns and variations in DNA methylation at single-base resolution.
• Regulatory, evolutionary, and disease studies: Support investigations of DNA methylation roles in regulatory processes, evolution, and disease within eukaryotic organisms.

Methodology:

Construction of annotated methylomes from publicly available whole-genome bisulfite sequencing (BS-seq) datasets and integration with MethPipe low- and high-level analysis pipelines.