Mizutama

Mizutama counts cells in photographs of blood smears by converting images into grayscale trinary representations and identifying erythrocytes and other blood cells for quantitative hematological profiling.


Key Features:

  • Input type: Operates on photographic images of blood smears.
  • Image conversion: Transforms original photographs into grayscale trinary images using a thresholding method.
  • Cell-background separation: Distinguishes cells from background via threshold-based trinary conversion.
  • Detection parameters: Searches for cells using size criteria and the relative dimensions of the nucleus compared to cytoplasm surface area.
  • Cell types counted: Identifies and counts erythrocytes and other blood cells.
  • Performance metric: Reports an approximate 1.4% failure rate in detecting avian red cells in standard microscopic photographs.
  • Processing speed: Performs image processing and cell detection rapidly.

Scientific Applications:

  • Hematological profiling: Provides quantitative counts for blood-cell-based hematological analyses.
  • Physiological studies: Enables large-scale erythrocyte and blood-cell quantification for physiological investigations.
  • Animal health assessment: Supports hematological assessments in animal health and veterinary studies.

Methodology:

Transforms photographic images into grayscale trinary images using a thresholding method and searches for cells based on size and the nucleus-to-cytoplasm surface area ratio.

Topics

Details

License:
Unlicense
Maturity:
Mature
Cost:
Free of charge
Tool Type:
desktop application
Operating Systems:
Linux, Windows, Mac
Added:
5/21/2019
Last Updated:
6/16/2020

Operations

Publications

Ochoa D, Redondo T, Moreno-Rueda G. Mizutama: A Quick, Easy, and Accurate Method for Counting Erythrocytes. Physiological and Biochemical Zoology. 2019;92(2):206-210. doi:10.1086/702666. PMID:30730249.