Mizutama
Mizutama counts cells in photographs of blood smears by converting images into grayscale trinary representations and identifying erythrocytes and other blood cells for quantitative hematological profiling.
Key Features:
- Input type: Operates on photographic images of blood smears.
- Image conversion: Transforms original photographs into grayscale trinary images using a thresholding method.
- Cell-background separation: Distinguishes cells from background via threshold-based trinary conversion.
- Detection parameters: Searches for cells using size criteria and the relative dimensions of the nucleus compared to cytoplasm surface area.
- Cell types counted: Identifies and counts erythrocytes and other blood cells.
- Performance metric: Reports an approximate 1.4% failure rate in detecting avian red cells in standard microscopic photographs.
- Processing speed: Performs image processing and cell detection rapidly.
Scientific Applications:
- Hematological profiling: Provides quantitative counts for blood-cell-based hematological analyses.
- Physiological studies: Enables large-scale erythrocyte and blood-cell quantification for physiological investigations.
- Animal health assessment: Supports hematological assessments in animal health and veterinary studies.
Methodology:
Transforms photographic images into grayscale trinary images using a thresholding method and searches for cells based on size and the nucleus-to-cytoplasm surface area ratio.
Topics
Details
- License:
- Unlicense
- Maturity:
- Mature
- Cost:
- Free of charge
- Tool Type:
- desktop application
- Operating Systems:
- Linux, Windows, Mac
- Added:
- 5/21/2019
- Last Updated:
- 6/16/2020
Operations
Publications
Ochoa D, Redondo T, Moreno-Rueda G. Mizutama: A Quick, Easy, and Accurate Method for Counting Erythrocytes. Physiological and Biochemical Zoology. 2019;92(2):206-210. doi:10.1086/702666. PMID:30730249.
DOI: 10.1086/702666
PMID: 30730249