NMR

NMR quantifies and dynamically monitors metabolites, proteins, and RNA within heterotypic biomolecular networks from single in vitro samples using nuclear magnetic resonance spectroscopy to enable kinetic and network-level analyses.

Key Features:

• Heterotypic Network Quantification: Uses in vitro nuclear magnetic resonance spectroscopy to observe and quantify metabolites, proteins, and RNA simultaneously within a single sample.
• Dynamic Monitoring Across Reaction Types: Supports monitoring of elementary reaction types including bimolecular interactions, catalysis, and unimolecular changes.
• High-Resolution Temporal Data Collection: Records high-density time series (e.g., over 35 time points with more than 170 observables per point) in an eight-reaction co-transcriptional RNA folding network.
• Multiplex Reaction Constant Determination: Enables simultaneous (multiplex) determination of multiple core reaction constants across a network.
• Revealing Unexpected Molecular Interactions: Reconstructs co-transcriptional RNA folding networks to reveal cross-talk between reactions and dynamic phase-separation phenomena involving distinct RNA-binding domains during transcription.

Scientific Applications:

• Systems biology network dynamics: Elucidates dynamics of biomolecular networks and regulatory mechanisms at the systems level.
• RNA folding and transcriptional regulation: Applies to study co-transcriptional RNA folding and transcriptional regulation with kinetic and network resolution.
• Integration with biochemical assays and 'omics': Integrates dynamic biochemical assay resolution with the multiplexing capabilities of 'omics' approaches for comprehensive analysis of complex biological processes.

Methodology:

In vitro nuclear magnetic resonance spectroscopy (Systems NMR) is applied to single samples to dynamically quantify metabolites, proteins, and RNA in co-transcriptional RNA folding networks over time.