P3BSseq

P3BSseq: Parallel Bisulfite Sequencing Data Processing Pipeline

P3BSseq performs automated analysis of bisulfite sequencing (BSseq) data for DNA methylation profiling, integrating trimming, alignment, annotation, bisulfite conversion quality assessment, and methylome file generation within a parallelized workflow.

Key Features:

• Parallel Processing: Implements parallel computing to reduce BSseq data analysis time.
• Resource Optimization: Minimizes CPU and memory usage across computational environments.
• Automated Workflow: Executes trimming, alignment, annotation, bisulfite conversion efficiency assessment, BED methylome file generation, and NIH-compliant report production.
• Library Support: Processes directional and non-directional libraries, single-end and paired-end reads, including Whole Genome BSseq (WGBS) and Reduced Representation BSseq (RRBS) datasets.

Scientific Applications:

• DNA Methylation Analysis: Generates standardized methylome outputs for developmental biology, cancer research, and other epigenetic studies requiring accurate methylation profiling.

Methodology:

The pipeline sequentially trims low-quality bases from sequencing reads, aligns reads to a reference genome, annotates genomic features, evaluates bisulfite conversion efficiency, and generates BED-format methylome files and standardized reports.