PhaSepDB

PhaSepDB curates a database of proteins associated with liquid-liquid phase separation (LLPS) and membraneless organelles to support identification and analysis of sequence features underlying LLPS.

Key Features:

• Curated protein collection: Contains 2,914 non-redundant proteins manually curated based on their localization in various organelles.
• Data sources: Entries are derived from published scientific literature and public databases.
• Entry annotations: Each protein entry includes detailed summaries, literature references, and specific sequence features indicative of LLPS behavior.
• Molecular signatures: Provides molecular signatures derived from known sequence characteristics to identify potential phase separation-related proteins across the human proteome.

Scientific Applications:

• Identification of LLPS-associated proteins: Facilitates discovery and cataloging of proteins involved in LLPS and formation of membraneless organelles.
• Sequence determinant analysis: Enables analysis of sequence features associated with LLPS to generate hypotheses about molecular determinants of phase separation.
• Proteome-wide screening: Supports screening the human proteome for candidate phase-separating proteins using derived molecular signatures.
• Mechanistic and disease research: Supports investigation of mechanisms of phase separation and its implications for cellular function and disease.

Methodology:

Data were manually curated from published literature and public databases, and molecular signatures were derived from known sequence characteristics; each entry includes annotated sequence features and literature references.