PhageXpress

PhageXpress integrates electrohydrodynamic nanomixing with nanopore sequencing to accelerate phage display selection and mapping of single-chain Fv (scFv) sequences for antibody discovery.

Key Features:

• Electrohydrodynamic Manipulation: Employs electrohydrodynamic nanomixing to enhance target binding and reduce non-specific interactions during phage display selection.
• High-Throughput Nanopore Sequencing: Uses Oxford Nanopore Technologies' MinION coupled with custom bioinformatics pipelines to sequence and map scFv sequences from selected phage particles.
• Efficiency and Speed: Enables single-round biopanning with approximately 74% mapping of anti-NS1 scFv sequences while using about 30-fold fewer phage particles compared to traditional multi-round methods.

Scientific Applications:

• Antibody Discovery: Rapid isolation and mapping of scFv clones for reconstruction into monoclonal antibodies for therapeutic and diagnostic development.
• Therapeutic and Diagnostic Biologics: Application in discovery and development workflows for biologics requiring bio-recognition molecules such as scFv.
• Viral Antigen Targeting: Validated on Dengue virus (DENV) non-structural protein 1 (NS1), where it isolated and mapped a substantial number of unique scFv clones.

Methodology:

Electrohydrodynamic nanomixing to enhance phage–antigen interactions; single-round biopanning for selection; and nanopore sequencing on Oxford Nanopore Technologies' MinION with custom bioinformatics pipelines for sequencing and mapping of scFv sequences.