PrAnCER

PrAnCER quantifies spatiotemporal gait parameters from contrast-enhanced video recordings to assess motor function changes in rodent disease models.

Key Features:

• Contrast-enhanced recordings: Uses a translucent floor that obscures objects not in contact with the surface to isolate and highlight paw prints in video recordings.
• Automated quantification: Processes recordings with software that automatically extracts and analyzes spatiotemporal gait parameters.
• Measured parameters: Outputs include stance duration, hind paw contact area, stride length, and other spatiotemporal gait metrics.

Scientific Applications:

• Parkinson's Disease (haloperidol) model: In rats dosed with haloperidol (0.15 mg/kg and 0.30 mg/kg), PrAnCER detected increased stance duration and hind paw contact area at 0.15 mg/kg and increased contact area and stride length at 0.30 mg/kg.
• Rodent motor function assessment: Quantitative evaluation of gait changes to study motor dysfunction across rodent disease models.

Methodology:

High-resolution videos of rats walking on a translucent floor are captured; the software processes these videos to extract and analyze gait parameters automatically, and results were compared with manual scoring.