ProtMiscuity

ProtMiscuity catalogs catalytic promiscuity in proteins by providing a manually curated database of experimentally characterized canonical and promiscuous enzyme activities.

Key Features:

• Curated dataset: Manually curated collection encompassing 88 different reactions across 57 proteins from 40 distinct organisms.
• Comprehensive annotations: Entries record canonical and promiscuous activities with reaction substrates, products, and kinetic parameters.
• Active site mapping: Active sites mapped to protein sequences and structures.
• Search and browse: Search and browse by protein name, organism, and descriptions of canonical and promiscuous reactions.
• External resource links: Cross-references to external biological and functional annotation resources.

Scientific Applications:

• Mechanistic investigation: Facilitate exploration of underlying mechanisms of promiscuous catalytic reactions using experimentally derived entries.
• Enzyme function studies: Support analysis of enzyme versatility and adaptability by comparing canonical and promiscuous activities.
• Literature validation and data retrieval: Enable validation of published findings and retrieval of hard-to-find information on catalytic promiscuity.

Methodology:

Manual curation of experimentally derived data, mapping of active sites to protein sequences and structures, and provision of links to external annotation resources.