RBPome

RBPome catalogs RNA-binding proteins (RBPs) identified by RNA interactome capture (RIC) and enables analysis of their modulation by hypoxia and HIF-signaling in Caenorhabditis elegans, including vhl-1 loss-of-function mutants.

Key Features:

• Global RBP catalog: Contains a detailed RBPome of more than 1,300 nematode RBPs, including 270 novel proteins identified by RNA interactome capture (RIC).
• HIF-signaling modulation: Profiles changes in the RBPome under hypoxic conditions and in vhl-1 loss-of-function mutants to link HIF-signaling with RBP dynamics.
• Data mining and visualization: Provides analytical support for mining and visualizing RIC-derived RBP datasets.
• Differential RNA-binding analysis: Highlights differences in RNA-binding that are independent of protein abundance, indicating condition-specific RNA-binding changes such as those induced by hypoxia.

Scientific Applications:

• Hypoxia response studies: Enables investigation of how hypoxia and HIF-signaling alter RBP composition and RNA interactions in C. elegans.
• Longevity and organismal survival: Supports studies into RBP roles in longevity mechanisms and organismal adaptation to low-oxygen conditions.
• Disease mechanism and therapeutic target discovery: Informs research on RBP involvement in disease progression and the identification of potential therapeutic targets relevant to hypoxia-related pathology.

Methodology:

RNA interactome capture (RIC) performed on wild-type Caenorhabditis elegans and vhl-1 loss-of-function mutants.