Rchimerism

Rchimerism automates quantification of donor–host chimerism from PCR-based short tandem repeat (STR) data to support monitoring of engraftment and detection of relapse after hematopoietic stem cell transplantation (HSCT).

Key Features:

• Automated informative loci selection: Automatically identifies informative short tandem repeat loci for chimerism testing.
• Chimerism percentage calculation: Calculates chimerism percentages from PCR-based STR data.
• Time efficiency: Reduces per-sample analysis time to under one minute compared with 20–80 minutes for manual methods.
• Accuracy and validation: Validated against manual calculations across 60 patient samples with 100% concordance.

Scientific Applications:

• Engraftment monitoring: Assessing donor engraftment after hematopoietic stem cell transplantation (HSCT).
• Relapse detection: Detecting early relapse of leukemia or lymphoma by identifying shifts in chimerism percentages.

Methodology:

Uses PCR amplification data of short tandem repeat loci followed by capillary gel electrophoresis, with automated selection and quantification of loci to compute chimerism percentages.