SEVA

SEVA provides a standardized plasmid vector architecture and repository (SEVA-DB 3.0) for selecting, assembling, and analyzing plasmid constructs to deconstruct and reconstruct complex prokaryotic phenotypes.

Key Features:

• Plasmid repository: SEVA-DB 3.0 maintains an expanded, canonical collection of SEVA plasmid vectors for genetic and genomic engineering of prokaryotes.
• SBOL availability: Canonical SEVA plasmids are provided in machine-readable SBOL (Synthetic Biology Open Language) format to enable interoperability with other platforms.
• Construct visualization: Integrated bioinformatic tools enable visualization of plasmid constructs.
• In silico DNA manipulation: Integrated tools support virtual manipulation of DNA segments within constructs.
• Plasmid behavior simulation: The resource supports simulations of plasmid behavior under various in vivo conditions.

Scientific Applications:

• Vector selection and assembly: Selection of optimal plasmid vectors for deconstructing and reconstructing complex prokaryotic phenotypes.
• Non-standard bacterial species engineering: Extending synthetic biology approaches to non-standard bacterial species.
• Chassis programming: Genetically programming new prokaryotic chassis.
• Research and biotechnology: Support for fundamental research and biotechnological applications in genetic engineering and synthetic biology.

Methodology:

Integration of bioinformatic tools for construct visualization and virtual DNA-segment manipulation; provision of plasmid sequences in SBOL format; and simulation of plasmid behavior under various in vivo conditions.