Smart-3SEQ

Smart-3SEQ quantifies gene expression from small and degraded RNA samples to enable transcriptome profiling of FFPE tissues and laser-capture microdissected (LCM) cells, including single cells.

Key Features:

• Enhanced Sensitivity and Accuracy: Quantifies transcript abundance with high precision from limited amounts of total RNA.
• Compatibility with FFPE Tissues: Processes RNA extracted from formalin-fixed paraffin-embedded (FFPE) tissues despite degradation and fixation artifacts.
• Integration with Laser-Capture Microdissection (LCM): Supports analysis of small cell populations or single cells isolated by LCM within their native tissue context.
• FFPE Single-Cell Profiling: Enables generation of distinct biological profiles from FFPE single cells for characterizing cellular phenotypes in complex tissues such as the tumor microenvironment.
• Cost-Effectiveness for Large-Scale Studies: Provides a scalable approach for extensive gene expression profiling experiments using limited or archived samples.

Scientific Applications:

• Gene expression profiling of limited samples: Enables transcriptome analysis of small, degraded, or low-input RNA samples, including clinical and archival material.
• Cellular phenotype discovery: Facilitates identification of novel cellular phenotypes, such as macrophage subtypes within the tumor microenvironment.
• Retrospective studies on archived clinical samples: Allows leveraging FFPE tissue repositories for retrospective genomic investigations.

Methodology:

Quantifies transcript abundance from RNA sequencing (RNA-seq) data derived from small or degraded RNA, including RNA from FFPE tissues and LCM-isolated samples.