TASBE Flow Analytics
TASBE Flow Analytics performs calibrated analysis of flow cytometry data to quantify cell fluorescence and size for synthetic biology applications.
Key Features:
- Calibration and Filtering: Implements multistage calibration and filtering workflows that calibrate experimental samples against controls for accurate quantitative measurement.
- High-Throughput Data Processing: Processes large-scale, high-throughput flow cytometry datasets to support scalable analysis.
- Quantitative Analysis and Comparison: Performs quantitative analyses and statistical comparisons across experimental samples using appropriate statistical methods.
- Automated Reporting: Generates calibrated data outputs, figures, diagnostic information, and machine-readable reports.
Scientific Applications:
- Synthetic Biology: Supports quantitative measurement of cell fluorescence and size in synthetic biology experiments.
- Flow Cytometry Calibration and Comparison: Enables calibrated and reliable comparisons across flow cytometry experiments and controls.
- Research and Biotechnology: Applicable to basic biological research and biotechnological development requiring precise single-cell fluorescence and size measurements.
Methodology:
Data processing includes ingestion of experimental samples and controls; multistage calibration and filtering workflows; quantitative statistical analyses and sample comparisons; and automated generation of calibrated outputs, figures, and diagnostic machine-readable reports.
Topics
Details
- License:
- GPL-3.0
- Maturity:
- Mature
- Cost:
- Free of charge
- Tool Type:
- library
- Operating Systems:
- Linux, Windows, Mac
- Programming Languages:
- MATLAB, Python
- Added:
- 8/9/2019
- Last Updated:
- 6/16/2020
Operations
Publications
Beal J, Overney C, Adler A, Yaman F, Tiberio L, Samineni M. TASBE Flow Analytics: A Package for Calibrated Flow Cytometry Analysis. ACS Synthetic Biology. 2019;8(7):1524-1529. doi:10.1021/acssynbio.8b00533. PMID:31053031.
PMID: 31053031
Funding: - Division of Computing and Communication Foundations: 1522074
- Defense Advanced Research Projects Agency: FA8750-17-C-0231, HR0011-10-C-0168, W911NF-11-054, W911NF-17-2-0098
- National Science Foundation: 8600021241
Documentation
Downloads
Links
Repository
https://github.com/TASBE/Issue tracker
https://github.com/TASBE/TASBEFlowAnalytics/issues